Development and Validation of New Method for Atenolol, Hydrochlorothiazide and Losartan potassium by RP-HPLC: Its Application to Routine Quality Control Analysis

Simple, rapid and sensitive UV, HPLC and Dissolution methods has been developed and validated for the analysis of Atenolol, Hydrochlorothiazide and Losartan potassium in tablet formulation. Best chromatographic resolution was achieved on a reverse-phase Phenomenex C18 column using acetonitrile: 50mM potassium dihydrogen ortho phosphate (pH 3.5) ratio 50:50 as mobile phase with a flow rate of 1mL/min and isocratic elution with a total run time of 14 minutes. Sulphadoxine was selected as internal standard. The retention time of Atenolol, Hydrochlorothiazide, Losartan potassium and Internal Standard was found to be 5.550, 3.280, 7.370 and 12.397 respectively. Detection of the multicompounds was carried out at 270nm. The present newly developed method was found to be accurate, precise and can be useful for routine Quality control analysis.